Neuroprotective effects of Oleiferin F on mitochondrial-mediated apoptosis in glutamate-induced HT22 cells

Abstract

Neuronal disease is caused by neuronal cell damage, and a high concentration of glutamate causes neuronal cell death. The purpose of this experiment was to confirm the protective effect of Oleiferin F (OF) on glutamate-induced apoptosis in HT22 cells. Oleiferin F inhibited the death of glutamate-induced HT22 cells in a dose-dependent manner as confirmed by annexin V/propidium iodide (PI) double staining. Oleiferin F reduced disruption of the mitochondrial membrane potential (ΔΨm), p53 expression, the Bax/Bcl-2 expression ratio and mitochondrial apoptosis-inducing factor (AIF) protein release. Oleiferin F also reduced levels of the cleaved form of caspase pathway proteins (PARP, caspase-9, caspase-3), which lead to apoptosis. These results reveal the mechanism underlying the neuroprotective effect of Oleiferin F in HT22 cells. Oleiferin F may be useful in preventing or treating neurological diseases.